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Diazo compounds, sometimes don't respond to Lassaigne's test for nitrogen

Diazo compounds (R—N N—R) do not respond to Lassaigne’s test because they lose nitrogen (N2) even at a moderate temperature. The presence of nitrogen in these compounds can be detected by heating the compound with cupric oxide and testing the gas evolved for nitrogen (N2). Hydrazine (NH2—NH2) also fails to give this test since it does not contain carbon.

Amides on heating with soda lime gives out ammonia. Ammonia can be detected by its smell and by tests with litmus paper and Nessler’s reagent.

Proteins on burning in Bunsen flame gives a smell of burning feathers.

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